Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. The mwd50 was designed for the most accurate discrimination of small pcr products, with 50 bp steps up to 500 bp. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and. How to dilute dna ladders andersen lab, 2016 invitrogen 1. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis.
Generuler 1 kb plus dna ladder, readytouse thermo fisher. Neb also offers various conventional doublestranded dna molecular weight markers with sizes up to 23 kb and markers for pulsedfield gel electrophoresis pfge ranging from 1. The trackit format combines the most popular dna ladders with loading buffer in a single tube. Egel 1 kb plus dna ladder thermo fisher scientific. Invitrogen trackit 1 kb plus dna ladder fisher scientific. There have been no changes to product formulation or specifications. Invitrogen egel dna ladders are formulated specifically for maximum performance on invitrogen egel precast agarose gels and are provided in readytoload formats.
Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen egel dna ladders are formulated specifically for maximum performance on invitrogen egel precast agarose gels and are provided in readytoload formats. If samples need to be diluted, use te or other buffer of minimal ionic strength. The ladder with 12 fragments starts at 100 bp, therefore being suitable even for small qpcr products, and goes. This product or its use is covered by at least one claim of u. For the convenience, the amounts of the 500, 1kbp and 5kbp fragments are adjusted to show greater intensities. Do not heat the trackit 1kb plus dna ladder before loading.
Use the neb 1 kb ladder for size markers between 500 bp and 10 kb. This format minimizes time spent thawing, diluting, and adding. I am guessing the two bands showing up in the lanes with samples are the 28s and 18s rna bands but they dont. Powerful tool for estimating molecular weight of linear, doublestranded dna fragments. The ladder consists of nine bands in even increments 0. Plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. We currently have two different kinds of ladder in the lab.
Trackit 1kb plus dna ladder thermo fisher scientific. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments. The 1 kb plus dna ladder is composed of 14 individual dna fragments. How can i prepare working solution with 1 kb dna ladder.
Ultraranger 1kb dna ladder 300bp 24000bp size determination of digested dna with high molecular weight more 100bp and 1 kb ladders are offered from our partner biovision. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The ladder is composed of fifteen chromatographypurified individual dna fragments in base pairs. Product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable cas number mixture, not applicable ec number mixture, not applicable relevant identified uses this product is a laboratory preparation for. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. The 10 bands of the ladder contain fragments following sizes. View more information about our dna stains technology. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. Two high intensity reference bands of 4 kb and 500 bp are included. It is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for.
The egel 1 kb plus dna ladder is formulated for use with egel ex agarose gels. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. Highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. This marker can be used with either agarose or polyacrylamide gels. The fastgene dna ladders were developed for different applications. Invitrogen 10787026 view more versions of this product. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels. Is it ok to use dna molecular weight marker in rna. Generuler 1 kb dna ladder thermo fisher scientific. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis.
Neb dna ladders are available in standard formats and in a variety of convenient, readytoload formats. Invitrogen trackit 1 kb plus dna ladder 100 applications. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Price pricing info supplier page view company product page. Invitrogen 250 bp dna ladder 75ug nucleic acid molecular. This product is available in quickload purple format enjoy the convenience of a readytoload format. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Use the invitrogen 1 kb plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. The 1 kb and 3 kb bands contain more dna to provide internal orientation. Generuler 1 kb plus dna ladder thermo fisher scientific. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp.
The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. Thermo scientific generuler 1 kb plus dna ladder, readytouse. The 1 kb plus dna ladder is a set of 14 easytoremember band sizes for use in qualitative agarose gel analysis ranging in size from 12 kb down to 100 bp. For easy reference on agarose gels, the 1,000bp band is two to threetimes brighter than the other bands in the ladder.
The ladder is supplied in readytoload form in gel loading buffer. The 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 250 bp to 10 kb. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. Dna ladder, 1 kb plus, thermo scientific j76714 alfa aesar. The 1 kb, 3 kb, and 6 kb bands contain more dna to provide internal orientation. The product is supplied in a solution of 10 mm trishcl, ph 8. Dna marker blue bp 1kb dna ladder readytouse dna marker bp 1kb dna ladder is designed for use as molecular weight standard for agarose gel electrophoresis features. The invitrogen 1 kb plus dna ladder thermo fisher scientific.
The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm tris ph 7. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. Prepared from a plasmid containing repeats of a 250bp dna fragment, the ladder consists of 14 blunt fragments ranging in length from 250 to 3,500bp at 250bp increments. Invitrogen trackit 1 kb plus dna ladder 100 applications nucleic acid molecular weight markers biochemicals and reagents. The egel 1 kb plus dna ladder contains the unique tracking dyes, xylene cyanol ff xcff and tartrazine.
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